Biological Macromolecules and Enzymes
Activity 1
Data Table 1
Test Tube ID Observations
Test tube 1
Water + iodine potassium iodide
Test tube 2
Water + potato starch + iodine potassium iodide
Test tube 3
Water + albumin solution + iodine potassium iodide
1. Describe the controls used in the starch test (Activity 1).
Activity 2
Data Table 2
Test Tube ID Observations
Test tube 1
Water + Biuret reagent
Test tube 2
Water + albumin solution + Biuret reagent
Test tube 3
Water + potato starch solution + Biuret reagent
Activity 3
Data Table 3
Test Tube ID Observation
Water
Vegetable or olive oil
Vinegar
Salad dressing
Mustard or ketchup
Unknown A
Unknown B
Unknown C
2. In the quick test for fats (Activity 3), is there a relationship between the size of the spot and the fat content of the food? Explain your answer.
Activity 4
Data Table 4
Test Tube ID Observation
Test tube 1a
Albumin + pepsin + HCl + Biuret reagent before incubation
Test tube 2a
Albumin + pepsin + water + Biuret reagent before incubation
Test tube 1b
Albumin + pepsin + HCl + Biuret reagent after incubation
Test tube 2b
Albumin + pepsin + water + Biuret reagent after incubation
3. In which test tube(s) did protein digestion occur (Activity 4)? How do you know?
Activity 5
Data Table 5
Cup ID
Observations
Cup 1
Cracker + distilled water + iodine-potassium iodide
Cup 2
Cracker + saliva + iodine-potassium iodide
4. What do you think would happen if the saliva treatment of the starch (cracker) was conducted under very cold conditions? Very hot conditions?
Photos
Photo 1
Insert the photo from Activity 1, step 5. The following should be indicated in this photo:
• Test Tube 1 – water
• Test Tube 2 – starch
• Test Tube 3 – albumin
Photo 2
Insert the photo from Activity 1, step 5 for your additional food samples. Indicate which food is in which test tube.
Photo 3
Insert the photo from Activity 1, step 5. The following should be indicated in this photo:
• Test Tube 1 – water
• Test Tube 2 – albumin
• Test Tube 3 – starch
Photo 4
Insert the photo from Activity 2, step 5 for your additional food samples. Indicate which food is in which test tube.
Photo 5
Insert the photo from Activity 3, step 8 of your group of fat test samples. Indicate which spot is for which food.
Photo 6
Insert the photo from Activity 4, step 6, The following should be indicated in this photo:
• Test Tube 1a
• Test Tube 1b
• Test Tube 2a
• Test Tube 2b
Photo 7
Insert the photo from Activity 4, step 11, The following should be indicated in this photo:
• Test Tube 1a
• Test Tube 1b
• Test Tube 2a
• Test Tube 2b
Photos 8 & 9
Insert the photos from Activity 5, step 9. The following should be indicated in these photos:
• Cup 1
• Cup 2