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🦠BIOTECHNOLOGY : PRINCIPLES AND PROCESS | Q&A❓️ | IMP.QUESTIONS
CHAPTER 11
BIOTECHNOLOGY :PRINCIPLES AND PROCESSES
1.Observe the figure given below :
- (a) Identify the process
- (b) Write the steps of this process 1 , 2 and 3
- (c) What is a primer?
- (d) Name the enzyme involved in this process .
- (e) Give its use in r-DNA technology.
2.Given diagram shows a technique used in r-DNA technology.
- (a) Identify the technique
- (b)What is the purpose of this technique in r-DNA technology ?
- (c) Name the stain used in this technique for visualizing DNA under UV-light.
- (d) How can you visualize the separated DNA fragments in this technique ?
- (e) What is elution ?
3..Observe the picture given below:
- (a) Identify the instrument in the figure.
- (b) Write any one use of this instrument.
- (c) Label A,B.C and D.
4.Observe the following figure:
- (a) Identify the figure.
- (b) What does (i) rop (ii) Bam HI
- (c) Identify the selectable markers present in it.
- (d)Explain the significance of ori’
5.The given figure shows a sequence of DNA that can be cut down by restriction endonuclease.
- a) Name the sequence.
- b) Write down the name of restriction endonuclease, which recognises the above sequence.
- c) Give its peculiarity.
- ECoR1 is a restriction endonuclease .What does E ,co, R, 1 represent ?
7.How can we make a cell competent to receive a foreign gene or DNA ?
8.Match the items of column A with B A B
- a) Cloning vector (i) Bioreactor
- b) Separation of DNA fragments (ii) Taq polymerase
- c) PCR (iii) Eectrophoresis
- d) Converts raw materials into specific products.
(iv) Hind II
(v) p BR 322
9.Recombinant DNA technology is a complex process which involves several steps. Write down the major steps in recombinant DNA technology?
- The discovery of restriction endonuclease is considered as milestone in the history of genetic engineering.
- a) What are restriction enzymes ?
- b) Which is the first discovered restriction endonuclease ?
11.Autonomously replicating circular extra-chromosomal DNA is?
12.Differentiate endonuclease and exonuclease.
