December 4, 2021Construct a µM product formed / unit time using the extinction coefficient for PNP and the Beer- Lambert Law and calculate the rate of reaction/alkaline phosphatase activity as either µmole product formed min–1 or nmole product formed min–1 at pH = 7.0. by Osman For pH = 8.0, construct a table like below for each [S] Abs.410nm 50 µM PNP 0.818 Time (seconds) D […]